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Furthermore, replacement of Lys-131, Arg-135, and Lys-150, which surround Cys-151, to Met residues has been shown to reduce the ability of tBHQ, SFN, and NO to induce ARE-driven gene expression, which is consistent with the hypothesis that these basic residues increase the reactivity of Cys-151 through the formation of a thiolate anion [253]
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