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While glutathione itself crosses this barrier poorly, the brain can synthesize glutathione from precursor amino acids, and systemic glutathione elevation may indirectly support brain glutathione status through various mechanisms including reduced oxidative stress on blood-brain barrier integrity and improved availability of glutathione precursors

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3.2 Post-translational modifications and protein stability of GPX4 The protein abundance and functional activity of GPX4 are not solely determined by transcriptional levels but are precisely regulated by a series of complex post-transcriptional and post-translational modification events, including but not limited to ubiquitination, interaction with specific proteins, and potential phosphorylation and glycosylation ( in vivo , demonstrating that p21 plays an important anti-ferroptotic role in OA progression by modulating GPX4 stability (Zheng et al., 2024)